THE INFLUENCE OF PHYTOHORMONES AND SODIUM SELENATE ON THE MITOTIC ACTIVITY OF APICAL
MERISTEMS OF RAPE GERMINANT ROOTS (BRASSICA NAPUS)

Katashov Denis Aleksandrovich, Postgraduate student, Penza State University
(Penza, 40 Krasnaya str.), earendil73@rambler.ru
Khryanin Viktor Nikolaevich, Doctor of biological sciences, professor, sub-department of botany, physiology
and biochemistry, Penza State University, (Penza, 40 Krasnaya str.), viktor.khryanin@gmail.com

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Background. It is known that mitotic activity of apical root meristems influences their growth rate. The aim of this paper is to show the dependence of the mitotic index of the nucleus as the centre of cell regulation and the exogenous effects of phytohormones and sodium selenate.
Materials and methods. As a research object we used rape germinant roots which were grown on filter paper in Petri dishes with phytohormone solutions indolylacetic acid (IAA, 5 mM), brassinolide (BS, 1 mM), gibberellic acid (GA, 10 mM), sodium selenate (Na2SeO4, 0,005 M). The mi-totic activity of the apical root meristem was estimated by cytological methods (Pausheva, 1974).
Results. The results of the present study demonstrate that the treatment of the rape seeds with IAA increased the mitotic index by 65,3%, with BS – by 25%, with Na2SeO4 – by 11,1%, GA slightly reduced the mitotic index, whereas 6-BAP inhibited the mitotic activity of the apical root meristems.
Conclusions. On the basis of the obtained results we conclude that IAA, BS and sodium se-lenate in the used concentrations stimulated the mitotic activity of the apical rape root meristems and thus they can be used for the intensification of root growth, the increase of their absorbing ability and plant production.

rape, phytohormone, sodium selenate, mitotic index.

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